The Adrenocorticotrophic Hormone (ACTH) is synthesized in the pituitary gland and stimulates the adrenal glands to release cortisol. The ACTH tests are used, usually with cortisol tests, to aid in diagnosing and monitoring conditions linked with excessive or deficient cortisol. By measuring the levels of ACTH and cortisol hormones, healthcare professionals can directly assess the endocrine malfunction, facilitating prompt and effective intervention.