The ACTH 60-minute test is performed by administering synthetic ACTH and measuring adrenocorticotrophic hormone levels after 60 minutes. The test helps healthcare professionals evaluate baseline adrenal gland function and adrenal response to synthetic ACTH stimulation by assessing levels at the 60th minute. It is an important adrenal function test, providing insights about the hypothalamic-pituitary-adrenal axis. A suboptimal increase in cortisol level indicates adrenal dysfunction, guiding treatment decisions.