The cortisol 180-minute test evaluates blood cortisol levels 180 minutes post-administration of synthetic adrenocorticotropic hormone (ACTH), aiding in the assessment of adrenal gland function. Deviations in cortisol levels at the 180-minute mark may indicate potential adrenal issues, such as Addison’s disease or adrenal insufficiency. Comprehensive interpretation, considering other clinical data, is crucial for a thorough assessment.