Testing the trough plasma levels of Gentamicin is essential for evaluating the drug’s potential toxicity. Gentamicin, an aminoglycoside antibiotic, is commonly prescribed for severe gram-negative infections across various age groups. Trough levels serve as indicators of nephrotoxicity, neurotoxicity, and ototoxicity. Healthcare professionals rely on this test to gauge the drug’s safety in patients receiving full therapeutic doses and those with renal impairment.