Anisakis, a nematode parasite common in raw or undercooked fish, has a major allergen, Ani s1. Upon ingestion, larvae can penetrate the gastrointestinal tract, triggering a type 1 hypersensitivity reaction. It can induce allergic responses such as abdominal pain, nausea, and sometimes severe anaphylaxis. Healthcare professionals use this test to determine allergic status and guide allergen-specific immunotherapy.

Important note: Minimum volume is 1 mL and 0.5 mL for every additional allergen.