Anise flowering plant seeds are commonly used as a spice. Anise seeds are allergenic in some individuals, stimulating a type 1 hypersensitivity reaction to proteins found in anise seeds. Exposure can happen through the consumption of seeds or through contact. It can lead to symptoms such as hives, itching, or anaphylaxis. Healthcare professionals use this test to determine allergic status and guide allergen-specific immunotherapy.

Important note: Minimum volume is 1 mL and 0.5 mL for every additional allergen.