Black pepper is a widely used spice. Piperine, the main alkaloid in black pepper, might act as an allergen, sometimes triggering type 1 hypersensitivity in susceptible individuals. The immune response produces IgE antibodies, causing gastrointestinal symptoms such as vomiting, abdomen pain, indigestion, atopy, and anaphylaxis in severe conditions. Healthcare professionals use this test to determine allergic status and guide allergen-specific immunotherapy.

Important note: Minimum volume is 1 mL and 0.5 mL for every additional allergen.