Casein, a major protein present in milk and cheese, acts as an allergen.  Sensitization can lead to type 1 hypersensitivity reactions, producing IgE antibodies. Clinically, these allergens can induce mild gastrointestinal symptoms, atopy, or occasionally severe anaphylaxis. Healthcare professionals use this test to determine allergic status and guide allergen-specific immunotherapy.

Important note: Minimum volume is 1 mL and 0.5 mL for every additional allergen.