Syphilis TP Confirmation Western Blot (WB) is used to confirm presence of antibodies to Treponema pallidum (TP), causative of syphilis. It helps to confirm Screening test in conjunction with Rapid Plasma Reagin test. Treponemal antigens blotted onto membrane is incubated with patient’s serum. Visualization of bound antibodies is done with detection method. Confirmation by WB is crucial for accurate diagnosis and treatment.