The Troponin I test assesses the troponin protein levels present in cardiac tissue. Troponin I (TnI) is a contractile protein that regulates actin-myosin coupling by inhibiting ATPase activity. Healthcare providers utilize this test to identify myocardial injuries, such as acute myocardial infarctions (MI) and re-infarctions, for early detection of perioperative MI, assessing infarct size, and diagnosing other significant cardiovascular diseases.