A thyroid stimulating hormone (TSH) level test is performed by healthcare professionals to evaluate thyroid gland function related to the main cause of hypothyroidism and screen newborns for congenital hypothyroidism, thyroid cancer, and inflammation. The measurement of plasma thyroid stimulating hormone concentration 120 minutes after challenge aids in the early screening process. By facilitating rapid testing, an effective treatment plan can be designed, and patient care could be enhanced in diverse healthcare settings.